Zika virus is an arthropod-borne flavivirus, which is transmitted primarily by mosquitoes of the Aedes genus, but can also be transmitted through sexual intercourse. The overall goal of these guidelines is to provide guidance and evidence-based recommendations about the prevention of sexual transmission of Zika virus. The absolute risks of different clinical complications of Zika virus are not fully known and the prevention measures may differ. Nevertheless, it is essential for individuals to have information about the risks of sexual intercourse as a mode of transmission in itself. These guidelines are informed by an update of the evidence underpinning the interim guidance and follow the requirements of the formal WHO guideline development process.